The Porziuncola Museum is located in the rooms that survived the construction of the great Papal Basilica of Santa Maria degli Angeli, on the initiative of some friars at the beginning of the twentieth century.

It is an integral part of the itinerary of pilgrims and tourists who visit the memories of Saint Francis and offers a stopover of greater understanding of the places of the Saint, making use of archive documents, models and graphic reconstructions. The rooms host in chronological succession the works that made the Museum famous.

The aim was to collect in a single environment the various works of art kept, at the time, in different spaces of the Sanctuary. On the occasion of the Jubilee of 2000, in particular, an exhibition itinerary was set up in chronological order, according to modern canons, so as to retrace the history of the Franciscan community.

Based on a thematic subdivision, the museum is made up of six exhibition rooms:

• Porziuncola in the pre-Franciscan period, at the origins of Franciscan spirituality
• Porziuncola during the Franciscan Observance
• Virgin Mary at Porziuncola
• Alessian Basilica
• The partial rebuilding of 1840
• Ancient Convent

Among the most significant works in the collection is the Crucifix by Giunta Pisano, dated to around 1236, considered the first example of a patiens Crucifix in Italy in a monumental cross.
Another noteworthy work is the Madonna del Latte, a valuable polychrome stone sculpture dating back to the end of the 14th century, originally placed in the small temple at the top of the Porziuncola façade.